B-Chromosomes and E-l Isozyme Activity in Mosaic Bulbs of Scilla autumnalis (Liliaceae)
نویسنده
چکیده
The electrophoretic patterns of esterase (E-l), alcohol dehydrogenase (ADH), and glutamate oxaloacetate transaminase (GOT) isozymes were studied in two Spanish populations of the lily Scilla autumnalis with Bchromosome carrying individuals. The E-l isozyme activity appears only in those individuals with B-chromosomes. None of the bulbs free of B’s show it. Five bulbs, mosaic for B-content, were identified. Electrophoretic analysis shows that these bulbs are characterised by mosaicism for E-l isozyme activity. An analysis of individual roots by both electrophoretic and cytological methods shows that tissue mosaicism for B-content correlates with tissue mosaicism for E-l isozyme activity. The electrophoretic analysis of different roots from bulbs heterozygous for the Est-1 locus indicates that the structural gene for E-l is not located on the B-chromosome itself. Rather there is a derepressor effect of Bs on E-l isozyme activity. Since ADH and GOT patterns are unaffected by the presence of B-chromosomes it is clear that they do not exhibit a generalised derepressor effect.
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